Optimasi Lama Fermentasi Ekoenzim Limbah Buah dan Sayur terhadap Pertumbuhan Vegetatif dan Kandungan Klorofil Brassica rapa L. var. chinensis

MINDA, Paskalia Kristina (2025) Optimasi Lama Fermentasi Ekoenzim Limbah Buah dan Sayur terhadap Pertumbuhan Vegetatif dan Kandungan Klorofil Brassica rapa L. var. chinensis. Undergraduate thesis, Universitas Katolik Widya Mandira.

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Abstract

The quality of eco-enzymes derived from organic waste—as an environmentally friendly waste-processing method and a potential source of plant nutrients—is strongly influenced by fermentation duration, which determines microbial activity, the formation of organic compounds, and nutrient availability for plants. Differences in fermentation length are assumed to produce eco-enzymes with varying characteristics, leading to diverse plant responses, particularly in growth performance and chlorophyll content as indicators of photosynthetic efficiency. Brassica rapa L. var. chinensis was used as the test plant due to its economic value and strong dependence on photosynthesis. This study aimed to analyze the effects of different fermentation durations of eco-enzymes on vegetative growth and chlorophyll content, as well as determine the optimal fermentation period for enhancing plant growth. The experiment was conducted using a Randomized Block Design consisting of four treatments: P0 (control/NPK), P1 (30-day fermented ecoenzyme), P2 (45-day fermented eco-enzyme), and P3 (60-day fermented ecoenzyme), each with six replications. The observed vegetative growth parameters included plant height, number of leaves, leaf area, stem circumference, shoot fresh weight, and root length. Chlorophyll a, b, and total chlorophyll were analyzed using a UV–Vis spectrophotometer. All data were subjected to normality and homogeneity tests, followed by one-way ANOVA and Duncan’s post hoc test. The results showed that the 60-day fermented eco-enzyme produced the highest values in most vegetative growth and chlorophyll parameters, although not all differences were statistically significant. The 45-day fermentation yielded the highest chlorophyll a value, while the 30-day fermentation resulted in the lowest plant responses. Physiologically, the extended fermentation period is associated with increased microbial activity and the accumulation of bioactive compounds that support plant growth. Therefore, a 60-day fermentation duration can be recommended as the optimal period for producing eco-enzymes capable of enhancing vegetative growth and chlorophyll content in Brassica rapa L. var. chinensis.

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