Perbandingan Kandungan Vinkristin dan Vinblastin dari Herba Tapak Dara Merah Muda dan Tapak Dara Putih (Catharanthus roseus L.)

MATILDA, Oktaviana (2025) Perbandingan Kandungan Vinkristin dan Vinblastin dari Herba Tapak Dara Merah Muda dan Tapak Dara Putih (Catharanthus roseus L.). Undergraduate thesis, Universitas Katolik Widya Mandira.

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Abstract

Abstract Catharanthus roseus L., commonly known as Madagascar periwinkle, is a medicinal plant that contains important anticancer alkaloids, namely vincristine and vinblastine. This study aims to quantitatively compare the levels of these two compounds in pink and white types of periwinkle herbs. The method used was maceration extraction with 96% ethanol, followed by analysis using Ultra Performance Liquid Chromatography–Quadrupole Time-of-Flight–Mass Spectrometry/Mass Spectrometry (UPLC–QTOF–MS/MS), which offers high sensitivity for trace-level compounds. The research stages included sample collection and preparation, 6×24-hour extraction, extract concentration using a fan, and quantitative analysis through sample injection into the chromatographic system and detection via Electrospray Ionization (ESI⁺). Results showed that the ethanol extract yield of pink periwinkle herbs was 36.34%, higher than the white type at 33.2%. UPLC–QTOF–MS/MS analysis identified vincristine and vinblastine in both extracts, with vincristine levels significantly higher in the pink type (60.24% area) compared to the white (28.03% area). Vinblastine was also detected in the pink extract (16.68%) but was absent in the white. This difference indicates that the pink type has a higher biosynthetic potential for vinca alkaloids. This research provides a valuable contribution to selecting promising medicinal plant types and supports the development of natural raw materials for anticancer therapy.

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